Cytogenetic Responses of Plant Root Meristematic Cells to the Arsenic (III) Contaminated Groundwater in Eastern Parts of Burdwan District, W.B., India

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Abhijit Bandyopadhyay

Abstract

Both geogenic and anthropogenic activities have resulted in arsenic pollution of groundwater especially in the
Eastern region of Burdwan district in West Bengal. Response of Allium cepa L. genetic material to the presence
of potential cytotoxic and genotoxic substances in aquatic environment was used to evaluate the arsenic
contaminated groundwater-induced genotoxicity and hormesis. For in situ monitoring of the cytotoxicity level,
the inhibition of mitosis in root meristematic cells was assayed. To study genotoxicity, micronucleus assay and
DNA purity assay (by A260/280 ratio) were used. Treatment for 4 days of newly developed roots of Allium cepa
L. with water samples with arsenic content 50 μg L-1 exhibited stimulation in mitotic activity whereas samples
with arsenic 1000 μg L-1 showed inhibition of mitotic activity apparently indicating hormesis. Inhibition of
mitosis was compared with the concentration dependent increase in generation of reactive oxygen species
(ROS), cell death, decrease in activity of antioxidative enzymes (catalase, superoxide dismutase), the
absorbance ratio (A260/280) of DNA, or micronuclei in root cells. These findings indicated that contaminated
groundwater depending on the magnitude of the arsenic concentration, might either be mitogenic or
mitostatic/toxic, which in turn has obvious implications in agriculture and human health.

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How to Cite
1.
Bandyopadhyay A. Cytogenetic Responses of Plant Root Meristematic Cells to the Arsenic (III) Contaminated Groundwater in Eastern Parts of Burdwan District, W.B., India. IJPE [Internet]. 31Aug.2015 [cited 11Apr.2021];1(01):79-4. Available from: https://ijplantenviro.com/index.php/IJPE/article/view/1173
Section
Review Article