Microbial Treatment of di (2-ethyl hexyl) Phthalate by Lysinibacillus xylanilyticus Isolated from Landfill Soil

Abstract

The bacterial strains were isolated from landfill soil contaminated with phthalate, collected from dump yard near Byepass, Patna. The
strain named T23 was chosen among the isolated strains due to its high efficacy towards the degradation. We observed the effects of
various environmental and chemical factors for optimising the conditions for degradation. The strain T23 was identified as Lysinibacillus
xylanilyticus based on its phenotypic as well as phylogenetic characteristics by performing 16S rRNA gene sequencing and for the
determination of the metabolic end products after degradation. Gas chromatography and mass spectrometry (GC-MS) analysis was done
and the degradable intermediates obtained were 7, 10, 13-Hexadecatrienoic acid, Cyclotrisiloxane, n-Hexadecanoic acid, Oleic Acid and
Erucic acid. The strain T23 showed maximum degradation at pH 8.5 and temperature was 10.5 and it could tolerate up to 0-15% NaCl.
Maximum degradation was exhibited at the carbon source, dextrose, and nitrogen source, casein. The 23 strain was having maximum
potential for degradation which can be used for various remediation purposes.