Optimize Biosorption Potential of Micrococcus luteus on Arsenic, Lead, and Mercury obtained from Coal mine

Abstract

The main aim of the study was to find the metal biosorption capability of Arsenic, lead and mercury resistant bacteria Micrococcus luteus was isolated from heavy metal contaminated soil of Mand coalfield Raigarh. Bacteria were screened for multiple heavy metal resistance capabilities through enumeration on LB agar containing 300 ppm Pb(NO₃)², NaAsO and HgCl₂ separately. M. luteus showed high biosorption ability against Pb (95.21%), As (90.23%) and Hg (80.67%) were analyzed using ICP-MS. The effect of pH, temperature and metal concentration on the biosorption potential was also determined. The optimum condition of lead, arsenic and mercury at pH 4.30, 4.80 and 5.0 respectively, temperature at 37^oC and metal concentration As (260mM/L), Hg (240mM/L) and Pb (340mM/L) by M. luteus was studied. The present study indicates that M. luteus removes arsenic, lead and mercury competently from soil contaminated with heavy metal and therefore further research should explore to remediation of soil contaminated with heavy metals