Identification and characterization of Azotobacter sp. screened from different agro-climatic zones in Telangana.
Main Article Content
Abstract
Declining soil quality due to overexploited used of chemical-based fertilizers, pesticides and insecticides have deleterious effect on soil health. This has resulted in decreased productivity. The use of azotobacter sp. as an alternative to the chemical based Agri-practice can be a remedial step to increase soil healthiness and in turn productivity. For the proposed research, soil sample from rhizosphere of Pigeon pea cultivated at Mahabubnagar and Medchal agroclimatic zone of Telangana. The Azotobacter sp. were isolated and maintained on Ashby’s mannitol agar medium. The Azotobacter sp. were evaluated by morphological, biochemical and molecular attributes. Biochemical tests and 16s rRNA sequencing revealed identification of strains as Azotobacter beijerinckii strain (BKPOU06TS) and Azotobacter tropicalis strain (BKPOU08TS). The 16s rRNA sequences of Azotobacter beijerinckii strain (BKPOU06TS) and Azotobacter tropicalis strain (BKPOU08TS) were submitted to GenBank with accession ID- OP536202 and OP536206 respectively
Article Details
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1.
Prawan K, Kumar BK, Imran MM, Eshwar C, Jayapaul K. Identification and characterization of Azotobacter sp. screened from different agro-climatic zones in Telangana. IJPE [Internet]. 2023Aug.8 [cited 2025Feb.23];9(01):39-44. Available from: https://ijplantenviro.com/index.php/IJPE/article/view/1441
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Research Articles
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References
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Yamamoto K, Toya S, Sabidi S, Hoshiko Y, Maeda T. Diluted Luria-Bertani medium vs. sewage sludge as growth media: comparison of community structure and diversity in the culturable bacteria. Appl Microbiol Biotechnol. 2021 May;105(9):3787-3798. doi: 10.1007/s00253-021-11248-4. Epub 2021 Apr 15. PMID: 33856534.
Yusminah Hala and Alimuddin Ali 2019 J. Phys.: Conf. Ser. 1244 012019.
Yusminah Hala 2020 IOP Conf. Ser.: Earth Environ. Sci. 484 012086
Zhang D, Kan X, Huss SE, Jiang L, Chen LQ, Hu Y. Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin. J Vis Exp. 2018 Aug 14;(138):56684. doi: 10.3791/56684. PMID: 30175990; PMCID: PMC6126798.
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Zuhaib, M., Ashraf, S., Musheer, N., Ali, M. (2019). Role of Rhizospheric Microbes in the Management of Phytopathogens. In: Ansari, R., Mahmood, I. (eds) Plant Health Under Biotic Stress. Springer, Singapore. https://doi.org/10.1007/978-981-13-6040-4_4
M. Ângelo Rodrigues, Laurindo Chambula Ladeira, Margarida Arrobas,Azotobacter-enriched organic manures to increase nitrogen fixation and crop productivity,European Journal of Agronomy, Volume 93,2018, Pages 88-94,ISSN 1161-0301, https://doi.org/10.1016/j.eja.2018.01.002.
Azcorn R, Barea JM. Synthesis of auxins, gibberellins and cytokinins by Azotobacter vinelandi and Azotobacter beijerinckii related to effects produced on tomato plants. Plant Soil. 1975;43:609–619.
Ben-David A, Davidson CE. Estimation method for serial dilution experiments. J Microbiol Methods. 2014 Dec;107:214-21. doi: 10.1016/j.mimet.2014.08.023. Epub 2014 Sep 7. PMID: 25205541.
Cowan S.T., Bergey‘s Manual of Determinative Bacteriology, Nature 162 (1948) 833.
Chan K, Wong PY, Yu P, Hardick J, Wong KY, Wilson SA, Wu T, Hui Z, Gaydos C, Wong SS. A Rapid and Low-Cost PCR Thermal Cycler for Infectious Disease Diagnostics. PLoS One. 2016 Feb 12;11(2):e0149150. doi: 10.1371/journal.pone.0149150. PMID: 26872358; PMCID: PMC4752298.
Sorty, A.M., Bitla, U.M., Meena, K.K., Singh, N.P. (2018). Role of Microorganisms in Alleviating Abiotic Stresses. In: Panpatte, D., Jhala, Y., Shelat, H., Vyas, R. (eds) Microorganisms for Green Revolution. Microorganisms for Sustainability, vol 7. Springer, Singapore. https://doi.org/10.1007/978-981-10-7146-1_6.
Dadook M, Mehrabian S, Salehi M, Irian S. Morphological, biochemical and molecular characterization of twelve nitrogen-fixing bacteria and their response to various zinc concentration. Jundishapur J Microbiol. 2014 Apr;7(4):e9415. doi: 10.5812/jjm.9415. Epub 2014 Apr 1. PMID: 25147702; PMCID: PMC4138622.
Dos Santos HRM, Argolo CS, Argôlo-Filho RC, Loguercio LL. A 16S rDNA PCR-based theoretical to actual delta approach on culturable mock communities revealed severe losses of diversity information. BMC Microbiol. 2019 Apr 8;19(1):74. doi: 10.1186/s12866-019-1446-2. PMID: 30961521; PMCID: PMC6454784.
Estiyar HK, Khoei FR, Behrouzyar EK. The effect of nitrogen biofertilizer on yield and yield components of white bean (Phaseolus vulgariscv. Dorsa). International Journal of Biosciences. 2014;4(11):217–222
Gandora V, Gupta RD, Bhardwaj KKR. Abundance of Azotobacter in great soil groups of North–West Himalayas. J Indian Soc Soil Sci. 1998;46(3):379–383.
Hartman D. Perfecting your spread plate technique. J Microbiol Biol Educ. 2011 Dec 1;12(2):204-5. doi: 10.1128/jmbe.v12i2.324. PMID: 23653767; PMCID: PMC3577269.
Hennequin JR, Blachere H. Recherches sur la synthese de phytohormones et de composes phenoliques par Azotobacter et des bacteries de la rhizosphere. Ann Inst Pasteur. 1966;3:89–102.
Jiménez DJ, Montaña JS, Martínez MM. Characterization of free nitrogen fixing bacteria of the genus Azotobacter in organic vegetable-grown Colombian soils. Braz J Microbiol. 2011 Jul;42(3):846-58. doi: 10.1590/S1517-83822011000300003. Epub 2011 Sep 1. PMID: 24031700; PMCID: PMC3768769.
Khare P, Raj V, Chandra S, Agarwal S. Quantitative and qualitative assessment of DNA extracted from saliva for its use in forensic identification. J Forensic Dent Sci. 2014 May;6(2):81-5. doi: 10.4103/0975-1475.132529. PMID: 25125913; PMCID: PMC4130022.
Lévai L, Szilvia V, Nóra B, et al. Can wood ash and biofertilizer play a role in organic agriculture? Agronomski Glasnic. 2008;3:263–271.
Lorenz TC. Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. J Vis Exp. 2012 May 22;(63):e3998. doi: 10.3791/3998. PMID: 22664923; PMCID: PMC4846334.
McPherson MR, Wang P, Marsh EL, Mitchell RB, Schachtman DP. Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments. J Vis Exp. 2018 Jul 24;(137):57932. doi: 10.3791/57932. PMID: 30102263; PMCID: PMC6126543.
Rosselli R, Romoli O, Vitulo N, Vezzi A, Campanaro S, de Pascale F, Schiavon R, Tiarca M, Poletto F, Concheri G, Valle G, Squartini A. Direct 16S rRNA-seq from bacterial communities: a PCR-independent approach to simultaneously assess microbial diversity and functional activity potential of each taxon. Sci Rep. 2016 Aug 31;6:32165. doi: 10.1038/srep32165. PMID: 27577787; PMCID: PMC5006002.
Sagar A, Sayyed RZ, Ramteke PW, Ramakrishna W, Poczai P, Al Obaid S, Ansari MJ. Synergistic Effect of Azotobacter nigricans and Nitrogen Phosphorus Potassium Fertilizer on Agronomic and Yieldtraits of Maize (Zea mays L.). Front Plant Sci. 2022 Aug 3;13:952212. doi: 10.3389/fpls.2022.952212. PMID: 35991457; PMCID: PMC9384888.
Sayers EW, Bolton EE, Brister JR, Canese K, Chan J, Comeau DC, Connor R, Funk K, Kelly C, Kim S, Madej T, Marchler-Bauer A, Lanczycki C, Lathrop S, Lu Z, Thibaud-Nissen F, Murphy T, Phan L, Skripchenko Y, Tse T, Wang J, Williams R, Trawick BW, Pruitt KD, Sherry ST. Database resources of the national center for biotechnology information. Nucleic Acids Res. 2022 Jan 7;50(D1):D20-D26. doi: 10.1093/nar/gkab1112. PMID: 34850941; PMCID: PMC8728269.
Sousa AM, Machado I, Nicolau A, Pereira MO. Improvements on colony morphology identification towards bacterial profiling. J Microbiol Methods. 2013 Dec;95(3):327-35. doi: 10.1016/j.mimet.2013.09.020. Epub 2013 Oct 9. PMID: 24121049.
Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994 Nov 11;22(22):4673-80. doi: 10.1093/nar/22.22.4673. PMID: 7984417; PMCID: PMC308517.
Tziachris P, Aschonitis V, Metaxa E, Bountla A. A soil parameter dataset collected by agricultural farms in northern Greece. Data Brief. 2022 Jun 23;43:108408. doi: 10.1016/j.dib.2022.108408. PMID: 35799851; PMCID: PMC9253687.
Wakarera PW, Ojola P, Njeru EM. Characterization and diversity of native Azotobacter spp. isolated from semi-arid agroecosystems of Eastern Kenya. Biol Lett. 2022 Mar;18(3):20210612. doi: 10.1098/rsbl.2021.0612. Epub 2022 Mar 23. PMID: 35317624; PMCID: PMC8941396.
Yamamoto K, Toya S, Sabidi S, Hoshiko Y, Maeda T. Diluted Luria-Bertani medium vs. sewage sludge as growth media: comparison of community structure and diversity in the culturable bacteria. Appl Microbiol Biotechnol. 2021 May;105(9):3787-3798. doi: 10.1007/s00253-021-11248-4. Epub 2021 Apr 15. PMID: 33856534.
Yusminah Hala and Alimuddin Ali 2019 J. Phys.: Conf. Ser. 1244 012019.
Yusminah Hala 2020 IOP Conf. Ser.: Earth Environ. Sci. 484 012086
Zhang D, Kan X, Huss SE, Jiang L, Chen LQ, Hu Y. Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin. J Vis Exp. 2018 Aug 14;(138):56684. doi: 10.3791/56684. PMID: 30175990; PMCID: PMC6126798.
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14.
Zuhaib, M., Ashraf, S., Musheer, N., Ali, M. (2019). Role of Rhizospheric Microbes in the Management of Phytopathogens. In: Ansari, R., Mahmood, I. (eds) Plant Health Under Biotic Stress. Springer, Singapore. https://doi.org/10.1007/978-981-13-6040-4_4